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To continue to learn from experts in the field of histology, it is now possible to access the Sakura Webinar Series to view webinars that you may have missed, or would like to revisit. In addition, webinars recorded after January 2012 will include a quiz and the opportunity to earn 1 contact hour certificate provided for ASCP Certification Maintenance. Watch and learn today.

Basic Scientific Principles of Molecular Pathology Techniques

Presented on June 7, 2013.

Presented by Ryan R. Fortna, MD, PhD, Northwest Pathology, Bellingham, WA.

Molecular pathology is quickly becoming an integral component of medicine, providing important diagnostic, prognostic, and therapy-related information as an adjunct to routine histopathology in a variety of situations. Some of these molecular techniques are becoming more commonly used in general anatomic pathology laboratories, including in-situ hybridization (ISH), various forms of polymerase chain reaction (PCR), mutation …

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Histology Process Improvement—Tissue Processing From Conventional Overnight to Continuous Rapid

Presented on April 18, 2013.

Presented by William DeSalvo, BS, HTL(ASCP) Production Manager, Sonora Quest Laboratories.

Process Improvement is essential for improving quality, increasing productivity, and reducing costs, and the right tissue processing protocol is essential for creating a successful integration of instrumentation and process workflow. The tried and true tissue processing methods used today in most histology labs will not support the precision demanded by introduction of new techniques required …

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In-Situ Hybridization Techniques on Paraffin-Embedded Tissue

Presented on March 21, 2013.

Presented by Laura Vivian, HT(ASCP)QIHC, Capital Digestive Care, Laboratory Division.

Enormous advancements in molecular technology have influenced the diagnosis and study of infectious diseases. Along with this has been an increased interest in the use of paraffin-embedded specimens for nucleic acid hybridization assays (in-situ hybridization). In-situ hybridization targets specific RNA and DNA sequences. Many viruses, bacteria, and other microorganisms can be localized by ISH. This …

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Validation of Breast Prognostic Markers using Image Analysis

Presented on October 25, 2012.

Presented by Lakshmi Alaparthi, MBE, Inova Health System.

Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) are well-established predictive markers for breast cancers and are routinely tested on patient’s tumor samples by immunohistochemical (IHC) study. As with all IHC studies of therapeutic targets, accurate and quantitative assessment of the results is critical and has a substantial impact on …

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Embracing Disruptive Innovations to Integrate Patient-centric Pathology Process Improvements

Presented on September 21, 2012.

Presented by Vince D'Mello, BSc, Grand River Hospital, St Mary's General Hospital and D'Mello LabMed Consultants.

An emerging global trend in clinical laboratories and health care is the increasing interest in adopting quality improvement tools to improve complex work processes. The outcome of engaging in such initiatives includes impressive results such as reduced patient waiting times, improved turnaround times, increased productivity, lower transactional costs for the service, and, most importantly, improved …

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Principles of Immunohistochemistry

Presented on August 16, 2012.

Presented by Laura Vivian, HT(ASCP)QIHC, Capital Digestive Care.

Immunohistochemistry (IHC) is microscopic localization of specific antigens (eg, proteins) in tissues by staining with antibodies labeled with fluorescent or pigmented material. Immunohistochemistry is used to determine the origin of a tumor as well as the prognosis and treatment. It is a critical technique used by the pathologist to assist in making a final diagnosis. …

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Standardization in the Gross Room, Start to Finish

Presented on June 14, 2012.

Presented by M. Lamar Jones, BS, HT(ASCP), Emory University Hospital.

Tissue specimens come into the gross room and must be accessioned and dissected for proper fixation. Some questions are: How do you standardize tissue sections? What tissue thickness is required to adequately fix and process tissue?  How do you select and implement the standardization process and maintain quality microscopic slides?  This webinar will provide tools …

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Cryotomy Techniques

Presented on May 24, 2012.

Presented by Diane Sterchi.

This seminar will cover a brief description of the equipment, ancillary equipment, and consumables used to perform cryotomy. The majority of the seminar will be devoted to cryotomy methods and alternatives, picking the correct temperature, embedding medium, freezing techniques, and the type of blades to use for different tissue types. This seminar should provide a …

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Personalized Medicine Demands a Precision Digital Process Model

Presented on April 19, 2012.

Presented by William DeSalvo, BS, HTL(ASCP), Sonora Quest Laboratories.

Personalized medicine to the patient means the histology lab has a new challenge to transform to a digital pathology lab. What role will the histotechnologist play in implementing and managing the digital solution? Once we understand the process and our role, we can start to change our current techniques to adapt to the demands of …

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Industrial Hygiene Monitoring in Histology Laboratories

Presented on March 8, 2012.

Presented by Jason Burrill.

Monitoring of indoor air quality produces data to make modifications to the work environment to reduce employee exposure to hazardous chemicals and can be a valuable tool to drive the use of engineering controls, modification of work practices and the institution of personal protective equipment. This presentation will cover the methods and strategies on how to conduct monitoring, determine …

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