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To continue to learn from experts in the field of histology, it is now possible to access the Sakura Webinar Series to view webinars that you may have missed, or would like to revisit. In addition, webinars recorded after January 2012 will include a quiz and the opportunity to earn 1 contact hour certificate provided for ASCP Certification Maintenance. Watch and learn today.

Going Where No One Has Gone Before—A Grass Roots Approach To Success In The Digital World

Presented on November 7, 2013.

Presented by Jesus Ellin, HT/PA ASCP BSBE, Yuma Regional Medical Center.

Anatomic pathology labs have a manufacturing-like workflow. The use of instruments is not new but requires upfront thinking about workflow and interoperability. Digital pathology, specifically whole slide imaging, is no different. Even in the digital age of whole slide imaging, we have the production of physical glass slides to reckon with. Therefore, the role of …

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A Systems Approach to H&E Staining

Presented on August 15, 2013.

Presented by Herbert Skip Brown, MDiv, HT(ASCP), Northwestern University.

Hematoxylin has been described as “the most valuable staining reagent used in histologic work.” While it is commonly known as the primary element of routine staining in histopathology, in effect it is a complex dye that demonstrates a wide variation of staining patterns depending on its interaction with other reagents and chemistries. Oftentimes various stain …

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IHC Controls—The Good, The Bad and The Ugly

Presented on July 18, 2013.

Presented by Wanda Jones, HT(ASCP), Immunohistochemistry Specialist, Celligent Diagnostics.

The proper selection and use of controls for IHC are critical to the assay, test, and patient diagnosis. Controls must be carefully selected, tested, and validated before use. Antibody specification sheets provide helpful guidance in the selection process of a positive control. The fixation, processing, and even microtomy of tissue for IHC controls play a …

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Basic Dynamics of Fixation and Processing

Presented on June 20, 2013.

Presented by Herbert Skip Brown, MDiv, HT(ASCP), Northwestern University.

Two of the most fundamentally critical elements of diagnostic histopathology are: 1) the ability to suspend all cellular activity in tissue and prevent degradation, and 2) to process that specimen in a manner that enhances subsequent steps such as microtomy and staining. The successful result of this is a microscopic image of cellular detail that …

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Basic Scientific Principles of Molecular Pathology Techniques

Presented on May 16, 2013.

Presented by Ryan R. Fortna, MD, PhD, Northwest Pathology, Bellingham, WA.

Molecular pathology is quickly becoming an integral component of medicine, providing important diagnostic, prognostic, and therapy-related information as an adjunct to routine histopathology in a variety of situations. Some of these molecular techniques are becoming more commonly used in general anatomic pathology laboratories, including in-situ hybridization (ISH), various forms of polymerase chain reaction (PCR), mutation …

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Histology Process Improvement—Tissue Processing From Conventional Overnight to Continuous Rapid

Presented on April 18, 2013.

Presented by William DeSalvo, BS, HTL(ASCP) Production Manager, Sonora Quest Laboratories.

Process Improvement is essential for improving quality, increasing productivity, and reducing costs, and the right tissue processing protocol is essential for creating a successful integration of instrumentation and process workflow. The tried and true tissue processing methods used today in most histology labs will not support the precision demanded by introduction of new techniques required …

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In-Situ Hybridization Techniques on Paraffin-Embedded Tissue

Presented on March 21, 2013.

Presented by Laura Vivian, HT(ASCP)QIHC, Capital Digestive Care, Laboratory Division.

Enormous advancements in molecular technology have influenced the diagnosis and study of infectious diseases. Along with this has been an increased interest in the use of paraffin-embedded specimens for nucleic acid hybridization assays (in-situ hybridization). In-situ hybridization targets specific RNA and DNA sequences. Many viruses, bacteria, and other microorganisms can be localized by ISH. This …

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Validation of Breast Prognostic Markers using Image Analysis

Presented on October 25, 2012.

Presented by Lakshmi Alaparthi, MBE, Inova Health System.

Estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2) are well-established predictive markers for breast cancers and are routinely tested on patient’s tumor samples by immunohistochemical (IHC) study. As with all IHC studies of therapeutic targets, accurate and quantitative assessment of the results is critical and has a substantial impact on …

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Embracing Disruptive Innovations to Integrate Patient-centric Pathology Process Improvements

Presented on September 21, 2012.

Presented by Vince D'Mello, BSc, Grand River Hospital, St Mary's General Hospital and D'Mello LabMed Consultants.

An emerging global trend in clinical laboratories and health care is the increasing interest in adopting quality improvement tools to improve complex work processes. The outcome of engaging in such initiatives includes impressive results such as reduced patient waiting times, improved turnaround times, increased productivity, lower transactional costs for the service, and, most importantly, improved …

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Principles of Immunohistochemistry

Presented on August 16, 2012.

Presented by Laura Vivian, HT(ASCP)QIHC, Capital Digestive Care.

Immunohistochemistry (IHC) is microscopic localization of specific antigens (eg, proteins) in tissues by staining with antibodies labeled with fluorescent or pigmented material. Immunohistochemistry is used to determine the origin of a tumor as well as the prognosis and treatment. It is a critical technique used by the pathologist to assist in making a final diagnosis. …

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